We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species.
- Authors
Villano, Clizia; Esposito, Salvatore; D'Amelia, Vincenzo; Garramone, Raffaele; Alioto, Daniela; Zoina, Astolfo; Aversano, Riccardo; Carputo, Domenico
- Abstract
Wild potatoes, as dynamic resource adapted to various environmental conditions, represent a powerful and informative reservoir of genes useful for breeding efforts. WRKY transcription factors (TFs) are encoded by one of the largest families in plants and are involved in several biological processes such as growth and development, signal transduction, and plant defence against stress. In this study, 79 and 84 genes encoding putative WRKY TFs have been identified in two wild potato relatives, Solanum commersonii and S. chacoense. Phylogenetic analysis of WRKY proteins divided ScWRKYs and SchWRKYs into three Groups and seven subGroups. Structural and phylogenetic comparative analyses suggested an interspecific variability of WRKYs. Analysis of gene expression profiles in different tissues and under various stresses allowed to select ScWRKY045 as a good candidate in wounding-response, ScWRKY055 as a bacterial infection triggered WRKY and ScWRKY023 as a multiple stress-responsive WRKY gene. Those WRKYs were further studied through interactome analysis allowing the identification of potential co-expression relationships between ScWRKYs/SchWRKYs and genes of various pathways. Overall, this study enabled the discrimination of WRKY genes that could be considered as potential candidates in both breeding programs and functional studies.
- Subjects
POTATOES; TRANSCRIPTION factors; EXONS (Genetics); PHYLOGENETIC models; CELLULAR signal transduction
- Publication
Scientific Reports, 2020, Vol 10, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-020-63823-w