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- Title
Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12.
- Authors
Oshima, Taku; Aiba, Hirofumi; Masuda, Yasushi; Kanaya, Shigehiko; Sugiura, Masahito; Wanner, Barry L.; Mori, Hirotada; Mizuno, Takeshi
- Abstract
Summary We have systematically examined the mRNA profiles of 36 two-component deletion mutants, which include all two-component regulatory systems of Escherichia coli , under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria–Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two-component systems. A high correlation coefficient of expression profile was found between several two-component mutants. Together, these results support the view that a network of functional interactions, such as cross-regulation, exists between different two-component systems. The compiled data are avail-able at our website ( http://ecoli.aist-nara.ac.jp/xp_analysis/ 2_components ).
- Subjects
ESCHERICHIA coli; DNA microarrays; GENE expression
- Publication
Molecular Microbiology, 2002, Vol 46, Issue 1, p281
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1046/j.1365-2958.2002.03170.x