We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Formosanin C suppresses cancer cell proliferation and migration by impeding autophagy machinery.
- Authors
Chu, Man‐Ling; Lin, Pei‐Wen; Liu, Yu‐Wen; Wu, Shan‐Ying; Lan, Sheng‐Hui; Su, Chun‐Li; Liu, Hsiao‐Sheng
- Abstract
Formosanin C (FC) is a natural compound extracted from Paris formosana Hayata with anticancer activity. FC induces both autophagy and apoptosis in human lung cancer cells. FC‐induced depolarization of mitochondrial membrane potential (MMP) may trigger mitophagy. In this study, we clarified the effect of FC on autophagy, mitophagy, and the role of autophagy in FC‐related cell death and motility. We found FC caused the continuous increase of LC3 II (representing autophagosomes) from 24 to 72 h without degradation after treatment of lung and colon cancer cells, indicating that FC blocks autophagic progression. In addition, we confirmed that FC also induces early stage autophagic activity. Altogether, FC is not only an inducer but also a blocker of autophagy progression. Moreover, FC increased MMP accompanied by overexpression of COX IV (mitochondria marker) and phosphorylated Parkin (p‐Parkin, mitophagy marker) in lung cancer cells, but no colocalization of LC3 with COX IV or p‐Parkin was detected under confocal microscopy. Moreover, FC could not block CCCP (mitophagy inducer)‐induced mitophagy. These results imply that FC disrupts mitochondria dynamics in the treated cells, and the underlying mechanism deserves further exploration. Functional analysis reveals that FC suppresses cell proliferation and motility through apoptosis and EMT‐related pathway, respectively. In conclusion, FC acts as an inducer as well as a blocker of autophagy that results in cancer cell apoptosis and decreased motility. Our findings shed the light on the development of combined therapy with FC and clinical anticancer drugs for cancer treatment.
- Subjects
PARIS (France); CANCER cell proliferation; CANCER cell migration; AUTOPHAGY; CELL motility; MEMBRANE potential; MITOCHONDRIAL pathology
- Publication
Kaohsiung Journal of Medical Sciences, 2023, Vol 39, Issue 5, p489
- ISSN
1607-551X
- Publication type
Article
- DOI
10.1002/kjm2.12658