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- Title
Stereoselective suppressive effects of protopanaxadiol epimers on UV-B-induced reactive oxygen species and matrix metalloproteinase-2 in human dermal keratinocytes.
- Authors
Oh, Sun-Joo; Lee, Sihyeong; Kho, Ye Eun; Kim, Kyunghoon; Jin, Chang Duck; Lim, Chang-Jin
- Abstract
This study aimed to assess the skin-related anti-photoaging activities of the 2 epimeric forms of protopanaxadiol (PPD), 20( S)-PPD and 20( R)-PPD, in cultured human keratinocytes (HaCaT cells). The anti-photoaging activity was evaluated by analyzing the levels of reactive oxygen species (ROS) and matrix metalloproteinases (MMPs), as well as cell viability for HaCaT cells under UV-B irradiation. The activities for MMP-2 and -1 in conditioned medium were determined using gelatin zymography, and MMP-2 protein in the conditioned medium was detected using Western blot analysis. 20( S)-PPD, but not 20( R)-PPD, suppressed UV-B-induced ROS elevation. Neither of the epimers, at the concentrations used, exhibited cytotoxicity, irrespective of UV-B irradiation. 20( S)-PPD, but not 20( R)-PPD, exhibited an inhibitory effect on UV-B-induced MMP-2 activity and expression in HaCaT cells. In brief, only 20( S)-PPD, a major metabolic product of PPD-type ginsenosides, inhibits UV-B-induced ROS and MMP-2 elevation, implying its stereospecific anti-photoaging activity on the skin.
- Subjects
STEREOSELECTIVE reactions; PHYSIOLOGICAL effects of ultraviolet radiation; REACTIVE oxygen species; MATRIX metalloproteinases; KERATINOCYTES
- Publication
Canadian Journal of Physiology & Pharmacology, 2015, Vol 93, Issue 1, p91
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2014-0273