We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Efficacy of TB-PCR using EBUS-TBNA samples in patients with intrathoracic granulomatous lymphadenopathy.
- Authors
Jung Seop Eom; Jeong Ha Mok; Min Ki Lee; Kwangha Lee; Min Ji Kim; Sun Mi Jang; Hae Jung Na; Seung Eon Song; Geewon Lee; Eun-Jung Jo; Mi-Hyun Kim; Ki Uk Kim; Hye-Kyung Park; Eom, Jung Seop; Mok, Jeong Ha; Lee, Min Ki; Lee, Kwangha; Kim, Min Ji; Jang, Sun Mi; Na, Hae Jung
- Abstract
<bold>Background: </bold>Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is widely used to perform mediastinal lymph node sampling. However, little information is available on polymerase chain reaction for Mycobacterium tuberculosis (TB-PCR) using EBUS-TBNA samples in patients with intrathoracic granulomatous lymphadenopathy (IGL).<bold>Methods: </bold>A retrospective study using a prospectively collected database was performed from January 2010 to December 2014 to evaluate the efficacy of the TB-PCR test using EBUS-TBNA samples in patients with IGL. During the study period, 87 consecutive patients with isolated intrathoracic lymphadenopathy who received EBUS-TBNA were registered and 46 patients with IGL were included.<bold>Results: </bold>Of the 46 patients with IGL, tuberculous lymphadenitis and sarcoidosis were diagnosed in 16 and 30 patients, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value of TB-PCR for tuberculous lymphadenitis were 56, 100, 100, and 81%, respectively. The overall diagnostic accuracy of TB-PCR for tuberculous lymphadenitis was 85%. In addition, seven (17%) patients had non-diagnostic results from a histological examination and all of them had non-diagnostic microbiological results of an acid-fast bacilli smear and culture. Four (57%) of the seven patients with non-diagnostic results had positive TB-PCR results, and anti-tuberculosis treatment led to clinical and radiological improvement in all of the patients.<bold>Conclusions: </bold>TB-PCR using EBUS-TBNA samples is a useful laboratory test for diagnosing IGL. Moreover, this technique can prevent further invasive evaluation in patients whose histological and microbiological tests are non-diagnostic.
- Subjects
LYMPHADENITIS; DIAGNOSTIC ultrasonic imaging; NEEDLE biopsy; POLYMERASE chain reaction; SENSITIVITY &; specificity (Statistics); TUBERCULOSIS; MYCOBACTERIUM tuberculosis; RETROSPECTIVE studies; SARCOIDOSIS diagnosis; TUBERCULOSIS diagnosis; LYMPH nodes; PREDICTIVE tests
- Publication
BMC Pulmonary Medicine, 2015, Vol 15, p1
- ISSN
1471-2466
- Publication type
journal article
- DOI
10.1186/s12890-015-0162-4