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- Title
Isolation, heterologous expression, and functional determination of an iron regulated transporter (IRT) gene involved in Fe<sup>2+</sup> transport and tolerance to Fe<sup>2+</sup> deficiency in Vitis vinifera.
- Authors
Song, Zhizhong; Wang, Xue; Li, Mengyuan; Ning, Youzheng; Shi, Shengpeng; Yang, Guangrong; Zhang, Hongxia; Tang, Meiling; Peng, Bin
- Abstract
In plants, iron (Fe) regulated transporters (IRT) play important roles in uptake and transport of Fe that contributes to plant growth and development. However, biological functions of IRT transporters in fruit trees are still unknown. This study isolated 10 VvIRT genes from ‘Marselan’ grape, with varying expression levels across different tissues/organs, particularly enhanced under Fe depletion, especially in roots. Notably, VvIRT7 is the most abundantly expressed IRT gene in grape, beneficially restoring the Fe2+ uptake defect of yeast mutant DEY1453. Furthermore, VvIRT7 showed dominant expression in the roots of irt1/35S::IRT7 complementation lines. Overexpressing of VvIRT7 rescued the retarded growth of irt1 knockout mutant, by increasing the fresh weight, dry weight, total root length, total root surface, lateral root numbers, total leaf chlorophyll, ACO activity, NiR activity, SDH activity, and tissue Fe concentration. This study provides insights for understanding molecular mechanisms of Fe uptake and transport in grape.Key message: A grape iron regulated transporter gene involved in Fe2+ transport and resistance to Fe2+ deficiency was isolated, heterologously expressed, and functionally characterized.
- Publication
Plant Cell, Tissue & Organ Culture, 2024, Vol 156, Issue 2, p1
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-023-02624-1