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- Title
Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model.
- Authors
Ren, Xian-Gang; Xue, Fei; Zhu, Yuan-Mao; Tong, Guang-Zhi; Wang, Yan-Hui; Feng, Jun-Ke; Shi, Hong-Fei; Gao, Yu-Ran
- Abstract
Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE−/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE−/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.
- Subjects
GENES; LABORATORY rabbits; ANIMAL models in research; GENE expression; FOOT &; mouth disease
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 8, p1159
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-9988-2