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- Title
One-step purification and characterization of an intracellular β-glucosidase from Metschnikowia pulcherrima.
- Authors
González-Pombo, Paula; Pérez, Gabriel; Carrau, Francisco; Guisán, José Manuel; Batista-Viera, Francisco; Brena, Beatriz M.
- Abstract
A collection of 60 non -Saccharomyces yeasts isolated from grape musts in Uruguayan vineyards was screened for β-glucosidase activity and Metschnikowia pulcherrima was the best source of this enzyme activity. Its major β-glucosidase was successfully purified to homogeneity by ion-exchange chromatography on amino-agarose gel. The enzyme exhibited an optimum catalytic activity at 50°C and pH 4.5 and was active against (1 → 4)-β and (1 → 2)-β glycosidic linkages. In spite of preserving 100% of its activity and stability in the presence of 12% (v/v) ethanol and 5 g glucose/l, the enzyme was unstable below pH 4. We characterized the β-glucosidase from M. pulcherrima with a view to its potential applications in wine-making.
- Subjects
SACCHAROMYCES; GLUCOSIDASES; ENZYME activation; HYDROGEN-ion concentration; LINKAGE (Genetics); GLUCOSE; YEAST; BIOTECHNOLOGY; SACCHAROMYCETACEAE
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 8, p1469
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9708-3