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- Title
Conserved regulatory elements in AMPK.
- Authors
Chen, Lei; Xin, Feng-Jiao; Wang, Jue; Hu, Jicheng; Zhang, Yuan-Yuan; Wan, Shuo; Cao, Lu-Sha; Lu, Chang; Li, Peng; Yan, S. Frank; Neumann, Dietbert; Schlattner, Uwe; Xia, Bin; Wang, Zhi-Xin; Wu, Jia-Wei
- Abstract
arising from B. Xiao et al. 472, 230-233 (2011)The AMP-activated protein kinase (AMPK), an αβγ heterotrimeric enzyme, has a central role in regulating cellular metabolism and energy homeostasis. The α-subunit of AMPK possesses the catalytic kinase domain, followed by a regulatory region comprising the autoinhibitory domain (AID) and α-linker. Structural and biochemical studies suggested that AID is central to mammalian AMPK regulation; however, this notion has been challenged recently by Xiao et al. on the basis of their active AMPK structure (Protein Data Bank accession 2Y94). On close inspection, however, we found that the α-subunit regulatory region was incorrectly built in their model, and our rebuilt model suggests a universal occurrence of the AID domain in AMPKs; we have also identified a novel regulatory motif that is essential for AMPK regulation.
- Subjects
PROTEIN kinases; HOMEOSTASIS; BIOCHEMISTRY; GENETIC regulation; METABOLISM
- Publication
Nature, 2013, Vol 498, Issue 7453, pE8
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature12189