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- Title
Genotoxicity of Motorcycle Exhaust Particles In Vivo and In Vitro.
- Authors
Yu Wen Cheng; Wen Wha Lee; Ching Hao Li; Chen Chen Lee; Jaw Jou Kang
- Abstract
We studied the genotoxic potency of motorcycle exhaust particles (MEP) by using a bacterial reversion assay and chromosome aberration and micronucleus tests. In the bacterial reversion assay (Ames test), MEP concentration-dependently increased TA98, TA100, and TA102 revertants in the presence of metabolic-activating enzymes. In the chromosome aberration test, MEP concentration-dependently increased abnormal structural chromosomes in CHO-K1 cells both with and without S9. Pretreatment with antioxidants (α-tocopherol, ascorbate, catalase, and NAC) showed varying degrees of inhibitory effect on the MEP-induced mutagenic effect and chromosome structural abnormalities. In the in vivo micronucleus test, MEP dose-dependently induced micronucleus formation in peripheral red blood cells after 24 and 48 h of treatment. The increase of micronucleated reticulocytes induced by MEP was inhibited by pretreatment with α-tocopherol and ascorbate. The fluorescence intensity of DCFH-DA–loaded CHO-K1 cells was increased upon the addition of MEP. Our data suggest that MEP can induce genotoxicity through a reactive oxygen species–(ROS-) dependent pathway, which can be augmented by metabolic activation. Alpha-tocopherol, ascorbate, catalase, and NAC can inhibit MEP-induced genotoxicity, indicating that ROS might be involved in this effect.
- Subjects
TOXICITY testing; ACHROMATISM; ANIMAL models of toxicology; MICROBIOLOGICAL assay; CHEMICAL inhibitors; MUTAGENICITY testing; FLUORIMETRY; VITAMIN synthesis
- Publication
Toxicological Sciences, 2004, Vol 81, Issue 1, p103
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfh173