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- Title
Detection of β-1-4-galactan in compression wood of Sitka spruce [Picea sitchensis(Bong.) Carrière] by immunofluorescence.
- Authors
Clemens Altaner; Adrian I. Hapca; J. Paul Knox; Michael C. Jarvis
- Abstract
Abstractβ-1-4-Galactan has been detected in Sitka spruce [Picea sitchensis(Bong.) Carrière] by an immunofluorescence procedure. The anti-β-1-4-galactan monoclonal antibody LM5 was found to bind to compression wood (CW) tissue as identified by cell morphology features. In cross-sections, year rings with very fine bands of galactan-containing tissue were found. Cells in these bands expressed no morphological CW features. This finding was interpreted to imply that β-1-4-galactan synthesis is one of the first physiological reactions of CW formation. In cases where the CW character was pronounced, galactan was exclusively located in the outer cell-wall layers and was absent from the majority of the inner S2 layer.
- Subjects
IMMUNOFLUORESCENCE; MONOCLONAL antibodies; GALACTANS; SITKA spruce; WOOD; FOREST products
- Publication
Holzforschung: International Journal of the Biology, Chemistry, Physics, & Technology of Wood, 2007, Vol 61, Issue 3, p311
- ISSN
0018-3830
- Publication type
Article
- DOI
10.1515/HF.2007.049