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- Title
The apoptotic effect of sarsasapogenin from Anemarrhena asphodeloides on HepG2 human hepatoma cells
- Authors
Bao, Wenna; Pan, Haifeng; Lu, Min; Ni, Yuan; Zhang, Rui; Gong, Xingguo
- Abstract
Abstract: Sarsasapogenin, a kind of mainly effective components of Anemarrhena asphodeloides Bunge (Liliaceae) has the effects of being anti-diabetes and improving memory. However, there are few reports focusing on its anti-tumor effects. In this study, the sarsasapogenin was extracted from rhizomes of A. asphodeloides Bunge and applied to inhibit HepG2 human hepatoma cells. MTT assay showed that sarsasapogenin induced a distinct dose- and time-dependent diminution of cell viability with IC50 of 42.4±1.0μg/ml for 48h. Furthermore, sarsasapogenin-induced apoptosis of HepG2 cells was verified by Hoechst 33258 staining, electron microscopy, DNA fragmentation and PI staining. Flow cytometry analysis showed that sarsasapogenin-induced cell apoptosis was through arrest of cell cycle in G2/M phase. Hence we proposed that sarsasapogenin could be used as an anti-liver cancer drug for future studies.
- Subjects
LIVER tumors; APOPTOSIS; ENDOCRINE diseases; ELECTRON microscopy
- Publication
Cell Biology International, 2007, Vol 31, Issue 9, p887
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2007.02.001