We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A New Diagnostic System in Cancer Research: Bioluminescent Imaging (BLI).
- Authors
D&idot;kmen, Z. Günnur; Gellert, Ginelle; Do&gcarron;an, Pakize; Mason, Ralph; Antich, Peter; Richer, Edmond; Wright, Woodring E.; Shay, Jerry W.
- Abstract
Bioluminescent imaging (BLI) is particularly well suited for imaging small animals, and it can be readily used by scientists who are already routinely using the luciferase gene as a reporter in cell-based assays. This strategy relies on ATP and an O2 dependent photochemical reaction between luciferin and luciferase, resulting in the release of photons from only live cells. In BLI, the intensity of the light detected by the device is proportional to the intensity of light emitted and the relationship is quantitative. This study was performed to determine the sensitivity of BLI for the imaging of lung metastasis and breast cancer models in addition to primary flank tumors. P549 lung cancer and MDA-MB 231 breast cancer cell lines were transfected with a lentiviral construct containing the luciferase gene and transfected cells were used as xenograft animal models. BLI was used to monitor tumor development and growth. Noninvasive bioluminescent imaging in vivo provides easy visualization of the tumor size and location so that imaging results can be used as an indicator of the treatment effectiveness, potentially accelerating the optimization of cancer treatment protocols.
- Subjects
CANCER research; BIOLUMINESCENCE assay; MEDICAL imaging systems; ADENOSINE triphosphate; CELLS; METASTASIS; LUNG cancer
- Publication
Turkish Journal of Medical Sciences, 2005, Vol 35, Issue 2, p65
- ISSN
1300-0144
- Publication type
Article