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- Title
Impact of Individual Viral Gene Segments from Influenza A/H5N8 Virus on the Protective Efficacy of Inactivated Subtype-Specific Influenza Vaccine.
- Authors
Moatasim, Yassmin; Kandeil, Ahmed; Mostafa, Ahmed; Kutkat, Omnia; Sayes, Mohamed El; El Taweel, Ahmed N.; AlKhazindar, Maha; AbdElSalam, Elsayed T.; El-Shesheny, Rabeh; Kayali, Ghazi; Ali, Mohamed A.; Abdel-Whab, El-Sayed Mohammed; Li, Xuguang
- Abstract
Since its emergence in 2014, the highly pathogenic avian influenza H5N8 virus has continuously and rapidly spread worldwide in the poultry sector resulting in huge economic losses. A typical inactivated H5N8 vaccine is prepared using the six internal genes from A/PR8/1934 (H1N1) and the two major antigenic proteins (HA and NA) from the circulating H5N8 strain with the HA modified to a low pathogenic form (PR8HA/NA-H5N8). The contribution of the other internal proteins from H5N8, either individually or in combination, to the overall protective efficacy of PR8-based H5N8 vaccine has not been investigated. Using reverse genetics, a set of PR8-based vaccines expressing the individual proteins from an H5N8 strain were rescued and compared to the parent PR8 and low pathogenic H5N8 strains and the commonly used PR8HA/NA-H5N8. Except for the PR8-based vaccine strains expressing the HA of H5N8, none of the rescued combinations could efficiently elicit virus-neutralizing antibodies. Compared to PR8, the non-HA viral proteins provided some protection to infected chickens six days post infection. We assume that this late protection was related to cell-based immunity rather than antibody-mediated immunity. This may explain the slight advantage of using full low pathogenic H5N8 instead of PR8HA/NA-H5N8 to improve protection by both the innate and the humoral arms of the immune system.
- Subjects
AVIAN influenza; INFLUENZA vaccines; VIRAL genes; INFLUENZA A virus; AVIAN influenza A virus; INFLUENZA viruses; INFLUENZA A virus, H1N1 subtype
- Publication
Pathogens, 2021, Vol 10, Issue 3, p368
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens10030368