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- Title
Magnetic Solid-Phase Extraction Using Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> Magnetic Nanoparticles Followed by UV-Vis Spectrometry for Determination of Paraquat in Plasma and Urine Samples.
- Authors
Chen, Li; Wang, Shujun; Sha, Ou; Wang, Yu; Yin, Xin; Chen, Xiaobing
- Abstract
A rapid and simple method was optimized and validated for the separation and quantification of paraquat, a frequently used herbicide and a leading cause of fatal poisoning worldwide, at trace levels with UV-Vis spectrophotometry in plasma and urine samples by direct magnetic solid-phase extraction. Fe3O4@SiO2 nanoparticles (NPs) were used as the magnetic solid-phase extraction agents and the paraquat absorbed on NPs was eluted using NaOH and ascorbic acid. Upon optimization, paraquat could be extracted and concentrated from various samples by 35-fold. The linear range, limit of detection (LOD), correlation coefficient (R), and relative standard deviation (RSD) could reach 15.0–400.0 μg/L, 12.2 μg/L, 0.9987, and 0.65% (n=5, c = 40.0 μg/L), respectively. The Fe3O4@SiO2 NPs could be reused up to five times. The method was successfully applied to the determination of paraquat in urine and plasma at different hemoperfusion numbers in a local hospital for the patient of paraquat poisoning. The experiment result could not only enable immediate medical intervention but also benefit patients’ survival.
- Subjects
PARAQUAT; SOLID phase extraction; MAGNETIC nanoparticles; IRON oxide nanoparticles; SILICA nanoparticles; ULTRAVIOLET-visible spectroscopy; BLOOD plasma; URINALYSIS
- Publication
Journal of Analytical Methods in Chemistry, 2017, p1
- ISSN
2090-8865
- Publication type
Article
- DOI
10.1155/2017/8704639