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- Title
The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis.
- Authors
Qihong Huang; Gumireddy, Kiranmai; Schrier, Mariette; le Sage, Carlos; Nagel, Remco; Nair, Suresh; Egan, David A.; Anping Li; Guanghua Huang; Klein-Szanto, Andres J.; Gimotty, Phyllis A.; Katsaros, Dionyssios; Coukos, George; Lin Zhang; Puré, Ellen; Agami, Reuven
- Abstract
MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that are important in many biological processes. Although the oncogenic and tumour-suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumour metastasis was addressed only recently and still remains largely unexplored. To identify potential metastasis-promoting miRNAs, we set up a genetic screen using a non-metastatic, human breast tumour cell line that was transduced with a miRNA-expression library and subjected to a trans-well migration assay. We found that human miR-373 and miR-520c stimulated cancer cell migration and invasion in vitro and in vivo, and that certain cancer cell lines depend on endogenous miR-373 activity to migrate efficiently. Mechanistically, the migration phenotype of miR-373 and miR-520c can be explained by suppression of CD44. We found significant upregulation of miR-373 in clinical breast cancer metastasis samples that correlated inversely with CD44 expression. Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs.
- Subjects
NON-coding RNA; CANCER invasiveness; METASTASIS; CANCER cells; CELL migration; CELLULAR control mechanisms
- Publication
Nature Cell Biology, 2008, Vol 10, Issue 2, p202
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1681