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- Title
Pharmacological advantages of melatonin in immunosenescence by improving activity of T lymphocytes.
- Authors
Yeong-Min Yoo; Su Kil Jang; Gwang-Hoon Kim; Jung-Youl Park; Seong-Soo Joo
- Abstract
Melatonin plays a critical role in regulating photoperiodic signals and has recently been shown to decrease immunosenescence with age. In this study, we examined whether melatonin activates T lymphocytes as major adaptive immune cells in in vitro and in vivo models. Splenocytes, CD4+, and naïve CD4 T lymphocytes were isolated from the spleen of BALB/c mice and the cell population patterns and mRNA profiles associated with T cell activation (CD28 and p21) and the melatonin receptor (MT1A and MT1B) were assessed. The T cell activationrelated proteins Ki67 and Bcl2 were also evaluated to confirm the relationship between gene and protein levels. Our data clearly revealed that CD28, p21, MT1A, and MT1B mRNAwere highly expressed in the presence of melatonin. Co-culture of CD4+ T lymphocyte and peritoneal macrophage 7 days after melatonin administration to young and aged mice significantly increased APRIL mRNA, suggesting induction or maintenance of T lymphocyte responses. We also found that the intracellular amount of Ki67 and Bcl2 proteins were significantly upregulated in aged CD4+ T lymphocytes, suggesting enhancing Tcell proliferation and ling-term maintenance of memory Tcells. Taken together, we conclude that melatonin supplementation may enhance immunity in aged individuals by upregulating immunosenescence indices in association with T lymphocytes and may be an attractive pharmacological candidate for aged and immunocompromised individuals.
- Subjects
MELATONIN; IMMUNOSENESCENCE; T cells
- Publication
Journal of Biomedical Research, 2016, Vol 30, Issue 4, p314
- ISSN
1674-8301
- Publication type
Article
- DOI
10.7555/JBR.30.2016K0010