We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp liner.
- Authors
LOW, Amy; YUSOF, Hamidah MOHD; REZA, Fazal; NURUL, Asma ABDULLAH; SRITHARAN, Shaminea; ALI, Niswathul HAANIA ZAIN; AZEEZ, Hasan SUBHI; HUSEIN, Adam
- Abstract
This in vitro study aimed to evaluate setting time and compressive strength of gypsum-based chitosan biomaterials and its effect on proliferation of stem cells from human exfoliated deciduous teeth (SHED) and alkaline phosphatase (ALP) activity. Pure-GYP was mixed with water (2.5 g: 1.9 mL); Gyp-CHT was prepared with gypsum, chitosan, and water (2.5 g: 0.285 g: 1.9 mL). Cell viability and ALP activity were assessed at different periods. Data were analyzed using SPSS (p<0.05). The setting times were 2.7 min and 2.8 min for pure-GYP and Gyp-CHT, respectively. Significantly higher compressive strength was observed with Gyp-CHT. SHED treatments with both materials were not cytotoxic. ALP was consistently higher in the treated groups compared with the control. Cellular attachments were evident with SEM. Excellent cellular viability with pure-GYP and Gyp-CHT, as well as increased ALP activities, suggested the possibility of tertiary dentin formation. Further studies are necessary to evaluate the biomaterials for its pulp protective potentialities.
- Subjects
BIOMATERIALS; DENTAL pulp cavities; CELL-mediated cytotoxicity; CHITOSAN; COMPRESSIVE strength
- Publication
Dental Materials Journal, 2015, Vol 34, Issue 4, p522
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2015-029