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- Title
Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds.
- Authors
Tanaka, R.; Ichioka, S.; Sekiya, N.; Ohura, N.; Uchino, S.; Ojima, A.; Itoh, Y.; Ishihara, O.; Nakatsuka, T.; Ikebuchi, K.
- Abstract
Background and Objectives The growth factors derived from platelets and plasma proteins mediate the wound-healing process that is characterized by the sequential migration and differentiation of several cell populations that give rise to angiogenesis, collagen synthesis, wound contraction, and re-epithelialization. To evaluate the efficacy of the blood-derived factors in wound healing, we examined a novel wound dressing consisting of concentrated human plasma proteins and platelet releasate (CPPP). Materials and Methods To generate CPPP, plasma proteins and platelets in the peripheral blood ( n = 5) were concentrated with the cold ethanol precipitation method. The thrombin obtained from the same blood unit and calcium chloride (CaCl2) were mixed to a concentrate. The CPPP has enough strength to dress cutaneous wounds and contains large amounts of cytokines and fibronectin. We applied the CPPP to excisional skin wounds in genetically healing-impaired model mice ( n= 5) and the wounds were evaluated 10 days after the operation. Results The area of CPPP-treated wounds decreased significantly compared with that of the control wounds (65% vs. 94% of the original size, respectively, P= 0·032). The immunostained section revealed a striking effect of CPPP on vascularization compared with the control wounds (13·2 vs. 2·7 vessels per mm2 as mean vascular density observed in the sections, respectively, P= 0·013). Conclusions Our results suggest that CPPP is a promising biologically active dressing for full-thickness skin wounds. CPPP can be an entirely autologous biological dressing, suggesting that it is free from the risk of transmission of pathogens through blood products.
- Subjects
GROWTH factors; BLOOD proteins; BLOOD platelets; ANIMAL models of wound healing; THROMBIN; BLOOD coagulation factors; CELL proliferation
- Publication
Vox Sanguinis, 2007, Vol 93, Issue 1, p49
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/j.1423-0410.2007.00913.x