We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Acetylshikonin promoting PI3K/Akt pathway and inhibiting SOX4 expression to delay intervertebral disc degeneration and low back pain.
- Authors
Huang, Yuming; Lei, Linchuan; Zhao, Zhuoyang; Li, Zemin; Wang, Hua; Chen, Shunlun; Zheng, Jinjian; Jiang, Guowei; Guo, Xingyu; Li, Jiamin; Wang, Jianru; Zheng, Zhaomin; Chen, Fan
- Abstract
This study investigated the molecular mechanism by which acetylshikonin inhibits SOX4 expression via the PI3K/Akt pathway to delay intervertebral disc degeneration (IVDD) and low back pain (LBP). Bulk RNA‐seq, RT‐qPCR, Western blot analysis, immunohistochemical staining, small interfering RNA (siSOX4), lentivirus (lentiv‐SOX4hi), and imaging techniques were used to assess SOX4 expression and validate its upstream regulatory pathway. Acetylshikonin and siSOX4 were injected into the IVD to measure IVDD. SOX4 expression significantly increased in degenerated IVD tissues. TNF‐α increased SOX4 expression and apoptosis‐related proteins in nucleus pulposus cells (NPCs). siSOX4 reduced TNF‐α‐induced NPCs apoptosis, while Lentiv‐SOX4hi increased it. The PI3K/Akt pathway was significantly correlated with SOX4, and acetylshikonin upregulated PI3K/Akt pathway while inhibiting SOX4 expression. In the anterior puncture IVDD mouse model, SOX4 expression was upregulated, and acetylshikonin and siSOX4 delayed IVDD‐induced LBP. Acetylshikonin delays IVDD‐induced LBP by inhibiting SOX4 expression through the PI3K/Akt pathway. These findings offer potential therapeutic targets for future treatments.
- Subjects
PI3K/AKT pathway; LUMBAR pain; INTERVERTEBRAL disk; GENE expression; SOX transcription factors
- Publication
Journal of Orthopaedic Research, 2024, Vol 42, Issue 1, p172
- ISSN
0736-0266
- Publication type
Article
- DOI
10.1002/jor.25653