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- Title
Inactivation of parvovirus B19 in coagulation factor concentrates by UVC radiation: assessment by an in vitro infectivity assay using CFU-E derived from peripheral blood CD34+ cells.
- Authors
Sugawara H; Motokawa R; Abe H; Yamaguchi M; Yamada-Ohnishi Y; Hirayama J; Sakata H; Sato S; Kamo N; Ikebuchi K; Ikeda H; Sugawara, H; Motokawa, R; Abe, H; Yamaguchi, M; Yamada-Ohnishi, Y; Hirayama, J; Sakata, H; Sato, S; Kamo, N
- Abstract
<bold>Background: </bold>Nonenveloped and thermostable viruses such as parvovirus B19 (B19) can be transmitted to patients who are receiving plasma-derived coagulation factor concentrates treated by the S/D method for inactivating enveloped viruses. Therefore, it is important to develop and validate new methods for the inactivation of nonenveloped viruses.<bold>Study Design and Methods: </bold>Suspensions of B19 in coagulation factor concentrates (FVIII) were irradiated with UVC light. B19 infectivity was determined by an indirect immunofluorescence assay using CFU-E, as a host cell, derived from peripheral blood CD34+ cells. The effects of catechins on B19 infectivity and on FVIII activity after UVC illumination were also examined.<bold>Results: </bold>The indirect immunofluorescence assay estimated the B19 infectivity of samples containing virus copies of 10(5) to 10(11) per 10 microL to be a median tissue culture-infectious dose of 10(0.3) to 10(5.4) per 10 microL. B19 was inactivated by 3 log at 750 J per m(2) of UVC radiation and was undetectable after 1000 or 2000 J per m(2) of irradiation. However, FVIII activity decreased to 55 to 60 percent of pretreatment activity after 2000 J per m(2) of UVC radiation. This was inhibited in the presence of rutin or catechins. Epigallocatechin gallate could maintain FVIII activity at almost 100 percent of pretreatment activity after 2000 J per m(2) of UVC radiation, while B19 infectivity was decreased to undetectable levels, which resulted in >3.9 log inactivation.<bold>Conclusion: </bold>UVC radiation in the presence of catechins, especially epigallocatechin gallate, appears to be an effective method of increasing the viral safety of FVIII concentrates without the loss of coagulation activity.
- Publication
Transfusion, 2001, Vol 41, Issue 4, p456
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1046/j.1537-2995.2001.41040456.x