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- Title
Immunochemical determination of cellular prion protein in plasma from healthy subjects and patients with sporadic CJD or other neurologic diseases.
- Authors
Völkel, Dirk; Zimmermann, Klaus; Zerr, Inga; Bodemer, Monica; Lindner, Thomas; Turecek, Peter L.; Poser, Sigrid; Schwarz, Hans P.; Völkel, D; Zimmermann, K; Zerr, I; Bodemer, M; Lindner, T; Turecek, P L; Poser, S; Schwarz, H P
- Abstract
<bold>Background: </bold>Creutzfeldt-Jakob disease is thought to be caused by conversion of cellular prion protein (PrP) from its soluble form (PrP(sen)) to a pathologic form (PrP(res)). The occurrence of a new variant of CJD has increased the demand for a rapid assay capable of detecting a theoretical risk of transmission of the disease by blood or plasma.<bold>Study Design and Methods: </bold>A quantitative sandwich ELISA for routine screening was developed for analysis of PrP levels in plasma. The time-resolved dissociation-enhanced fluorescence technology allowed a detection limit in plasma samples of approximately 50 pg/mL. Levels of PrP(sen) were tested in plasma from 31 patients with CJD, from 11 patients with other neurodegenerative diseases, and from a control group of 200 healthy subjects.<bold>Results: </bold>The assay recognized both PrP(sen) and pathologic PrP(res), but did not differentiate between the two isoforms. PrP(sen) levels were higher in plasma from both patient groups than in plasma from the control group: 27 of the 31 (87%) CJD patients and all patients with other neurodegenerative diseases had higher levels than the highest concentration found in the control group. No correlation was found between age and PrP level. No signal could be detected in the CJD samples after protease K digestion, indicating that all detected PrP was protease-sensitive and therefore not pathologic.<bold>Conclusion: </bold>These data suggest that soluble PrP(sen) in plasma samples might be useful as a surrogate marker for a broad spectrum of neurologic diseases as well as for CJD.
- Subjects
CHRONIC wasting disease; PRION diseases
- Publication
Transfusion, 2001, Vol 41, Issue 4, p441
- ISSN
0041-1132
- Publication type
journal article
- DOI
10.1046/j.1537-2995.2001.41040441.x