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- Title
Effect of GM-CSF on cytokine induction by soluble β-glucan SCG in vitro in β-glucan-treated mice.
- Authors
Hida, Toshie H.; Kawaminami, Hiromi; Ishibashi, Ken-ichi; Miura, Noriko N.; Adachi, Yoshiyuki; Yadomae, Toshiro; Ohno, Naohito
- Abstract
SCG is a 6-branched 1,3-β-d-glucan, which are major cell wall structural components in fungi. Leukocytes from DBA/1 and DBA/2 mice are highly sensitive to SCG, producing cytokines such as GM-CSF, IFN-γ, TNF-α and IL-12p70, but not IL-6. GM-CSF plays a key biological role in this activity. In the present study, we examined the effect of giving i.p. SCG to DBA/2 mice on cytokine production in vitro. SCG was given i.p. to DBA/2 mice on day 0. Splenocytes were prepared on day 7 and cultured in the presence of SCG in vitro. The levels of cytokine production induced by SCG in vitro were lower in the cells from SCG-treated mice than in control mice. Expression of the β-glucan receptor, dectin-1, in SCG-treated mice was comparable with that shown in control mice. However, the consumption of exogenously added rmGM-CSF in vitro was observed in SCG-treated mice. The addition of a large amount of rmGM-CSF to the culture medium resulted in larger amounts of TNF-α and IL-6 in SCG-treated mice than in normal mice. These results suggested that GM-CSF was closely related with the reactivity of β-glucan. Giving SCG increased the number of macrophages and granulocytes in the spleen. These results suggested that in SCG-treated mice, a change of cell population would be related to modulation of the profile of cytokine production induced by SCG in vitro.
- Subjects
GLUCANS; CYTOKINES; FUNGI; LABORATORY mice; MACROPHAGES; GRANULOCYTES
- Publication
Microbiology & Immunology, 2009, Vol 53, Issue 7, p391
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2009.00139.x