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- Title
Protein kinase C ε phosphorylates keratin 8 at Ser8 and Ser23 in GH<sub>4</sub>C<sub>1</sub> cells stimulated by thyrotropin-releasing hormone.
- Authors
Akita, Yoshiko; Kawasaki, Hiroshi; Imajoh-Ohmi, Shinobu; Fukuda, Hiroyuki; Ohno, Shigeo; Hirano, Hisashi; Ono, Yoshitaka; Yonekawa, Hiromich
- Abstract
Protein kinase C ε (PKCε) is activated by thyrotropin-releasing hormone (TRH), a regulator of pituitary function in rat pituitary GH4C1 cells. We analyzed the downstream mechanism after PKCε activation. Exposure of GH4C1 cells to TRH or a phorbol ester increased the phosphorylation of three p52 proteins (p52a, p52b and p52c) and decreased the phosphorylation of destrin and cofilin. GF109203X, an inhibitor of protein kinases including PKC, inhibited phosphorylation of the p52 proteins by TRH stimulation. Peptide mapping, amino-acid sequencing, and immunochemical studies indicated that p52a, p52b, and p52c are the differentially phosphorylated isoforms of keratin 8 (K8), an intermediate filament protein. The unphosphorylated K8 (p52n) localized exclusively to the cytoskeleton, whereas the phosphorylated forms (especially p52c), which are increased in TRH-stimulated cells, localized mainly to the cytosol. K8 phosphorylation was enhanced in PKCε-overexpressing clones, and purified recombinant PKCε directly phosphorylated K8 with a profile similar to that observed in TRH-stimulated cells. PKCε and K8 colocalized near the nucleus under basal conditions and were concentrated in the cell periphery and cell–cell contact area after TRH stimulation. MS analyses of phospho-K8 and K8-synthesized peptide (amino acids 1–53) showed that PKCε phosphorylates Ser8 and Ser23 of K8. Phosphorylation of these sites is enhanced in TRH-stimulated cells and PKCε-overexpressing cells, as assessed by immunoblotting using antibodies to phospho-K8. These results suggest that K8 is a physiological substrate for PKCε, and the phosphorylation at Ser8 and Ser23 transduces, at least in part, TRH–PKCε signaling in pituitary cells.
- Subjects
PROTEIN kinase C; THYROTROPIN releasing factor; PITUITARY gland; PHORBOL esters; PHOSPHORYLATION; PEPTIDES
- Publication
FEBS Journal, 2007, Vol 274, Issue 13, p3270
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2007.05853.x