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- Title
The selective COX-2 inhibitor Etoricoxib reduces acute inflammatory markers in a model of neurogenic laryngitis but loses its efficacy with prolonged treatment.
- Authors
Lima-Rodrigues, Manuel; Lamas, Nuno; Valle-Fernandes, Ana; Cruz, Andrea; Vieira, Artur; Oliveira, Pedro; Pedrosa, Jorge; Castro, António; Reis, Rui; Baltazar, Fátima; Almeida, Armando
- Abstract
A randomised experimental study was used to evaluate the therapeutic effect of a selective cyclooxygenase-2 (COX-2) inhibitor in neurogenic laryngitis. Male Wistar Han rats were subjected to the nasogastric intubation model (NGI) of laryngitis for 1 and 2 weeks. The NGI animals were divided into three groups: (1) treated with COX-2 inhibitor Etoricoxib, (2) vehicle and (3) non-intubated animals. A fourth group of animals was submitted to NGI only. Laryngeal sections were immunostained for substance P (SP) and calcitonin gene-related peptide (CGRP) fibre-immunoreactivity (IR) and quantification of COX-2 positive cells through stereological analysis. The expression of COX-2, interleukins IL-1β, IL-6, IL-10 and tumour necrosis factor-α (TNF-α) was determined by quantitative real time QRT-PCR. Etoricoxib (6 mg/kg/day) was prepared in 0.9% sterile saline with 5% glucose (vehicle) and administered daily during 1 or 2 weeks. Treatment for 1 week with Etoricoxib attenuated the CGRP-IR fibre depletion, the COX-2-IR increased cell number and the TNF-α and COX-2 mRNA increased levels induced by NGI. Two weeks of treatment had no beneficial effect. Etoricoxib is effective in neurogenic laryngitis for limited periods of administration, indicating that selective COX-2 inhibitors should be evaluated in the future.
- Subjects
CYCLOOXYGENASE 2 inhibitors; LARYNGITIS; INTUBATION; STEREOLOGY; POLYMERASE chain reaction; MESSENGER RNA; TUMOR necrosis factors; CYTOKINES; DRUG efficacy; THERAPEUTICS
- Publication
Inflammation Research, 2010, Vol 59, Issue 9, p743
- ISSN
1023-3830
- Publication type
Article
- DOI
10.1007/s00011-010-0185-5