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- Title
Effects of the Water-Miscible Organic Solvents on Lactoperoxidase Purified from Creek-Water Buffalo Milk.
- Authors
Özdemir, H.; Hacibeyoglu, H. I.; Käfrevioglu, Ö. I.
- Abstract
Water buffalo lactoperoxidase (WBLPO) was purified with Amberlite CG-50 (NH4+ form) resin, CM-Sephadex C-50 ion-exchange chromatography, and Sephadex G-100 gel-filtration chromatography from skimmed buffalo milk. The purity of the WBLPO was shown with SDS-PAGE. The Rz(A412/A280) value for the WBLPO was 0.9. The optimum pH for the WBLPO was at 6.0. The Km value at optimum pH and 25°C was 0.13 mM. The Vmax value at optimum pH and 25°C was 5.3 μmol/min per ml. The Ki values for methanol, ethanol, dimethyl sulfoxide (DMSO), acetonitrile, isopropanol, tetrahydrofuran (THF), N,N"-dimethylformamide (DMF), and ethylene glycol were 1.087, 0.364, 0.302, 0.459, 0.330, 0.126, 0.093, and 2.125 M, respectively. All the solvents showed competitive inhibition. The I50 values of methanol, ethanol, dimethyl sulfoxide, acetonitrile, isopropanol, tetrahydrofuran, N,N"-dimethylformamide, and ethylene glycol were 2.910, 0.942, 0.537, 1.320, 0.875, 0.470, 0.405, and 3.920 M, respectively. Ethylene glycol, methanol, acetonitrile, and ethanol have been found to be very promising solvents for performing biocatalytic reactions with LPO in organic media.
- Subjects
ORGANIC solvents; ENZYMES; WATER buffalo; OXIDATION-reduction reaction; CHEMICAL reactions; BIOCHEMISTRY
- Publication
Biochemistry (00062979), 2003, Vol 68, Issue 3, p329
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1023/a:1023062402300