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- Title
Chromosome Karyotype and G-band Analysis of Bovine Small Intestinal Epithelial Cell Line.
- Authors
WANG Xueying; MENG Sudan; HE Lei; ZHAO Shujuan; WANG Conghui; LI Rishun; QIAN Weifeng; ZHANG Cai
- Abstract
In order to identify the genetic stability in biology of bovine small intestinal epithelial cell line (BIECs-21), BIECs-21 cells were cultured in vitro by using adherent culture, and chromosome and G-band samples were prepared to analyze the chromosome karyotype and G-band of BIECs-21 cells. The results show that the number of chromosomes in BIECs-21 cells is 2n = 60, including 29 pairs of autosomes and 1 pair of sex chromosomes (X and Y chromosomes). Among them, 29 pairs of autosomes are all terminal centromere chromosomes, with X chromosome being the larger centromere chromosome and Y chromosome being the smaller centromere chromosome in the sex chromosome. There is no significant difference in the number and position of G-bands pattern on the autosomes of BIECs-21 cells, except for X and Y sex chromosome. Compared with the normal chromosome karyotype of Holstein cattle, there is no significant change in the number and morphological structure of chromosomes in BIECs-21 cells, indicating that the genetic characteristics of the cell line are stable, and it can be used for the research on the molecular mechanisms of bovine intestinal related diseases.
- Subjects
KARYOTYPES; SEX chromosomes; CHROMOSOME structure; CHROMOSOMES; Y chromosome; X chromosome
- Publication
Journal of Henan University of Science & Technology, Natural Science, 2024, Vol 45, Issue 4, p73
- ISSN
1672-6871
- Publication type
Article
- DOI
10.15926/j.cnki.issn1672-6871.2024.04.010