We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nuclear Hsp104 safeguards the dormant translation machinery during quiescence.
- Authors
Kohler, Verena; Kohler, Andreas; Berglund, Lisa Larsson; Hao, Xinxin; Gersing, Sarah; Imhof, Axel; Nyström, Thomas; Höög, Johanna L.; Ott, Martin; Andréasson, Claes; Büttner, Sabrina
- Abstract
The resilience of cellular proteostasis declines with age, which drives protein aggregation and compromises viability. The nucleus has emerged as a key quality control compartment that handles misfolded proteins produced by the cytosolic protein biosynthesis system. Here, we find that age-associated metabolic cues target the yeast protein disaggregase Hsp104 to the nucleus to maintain a functional nuclear proteome during quiescence. The switch to respiratory metabolism and the accompanying decrease in translation rates direct cytosolic Hsp104 to the nucleus to interact with latent translation initiation factor eIF2 and to suppress protein aggregation. Hindering Hsp104 from entering the nucleus in quiescent cells results in delayed re-entry into the cell cycle due to compromised resumption of protein synthesis. In sum, we report that cytosolic-nuclear partitioning of the Hsp104 disaggregase is a critical mechanism to protect the latent protein synthesis machinery during quiescence in yeast, ensuring the rapid restart of translation once nutrients are replenished. During aging, proteins are damaged and can misfold, compromising cellular viability. Here, Kohler et al. uncover how aging cells maintain fitness by redirecting the protein repair factor Hsp104 to the nucleus in response to metabolic cues.
- Subjects
PROTEIN synthesis; CELLULAR aging; CELL nuclei; QUALITY control; CELL cycle; SEED dormancy
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-44538-8