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- Title
Improvement in Labeling Efficiency of Hydrophilic and Hydrophobic Fluorescent Molecules in Thick Tissue Sections.
- Authors
Tseng, Po-Hang; Chiang, Shu-Jen; Chen, Shean-Jen; Dong, Chen-Yuan
- Abstract
In this work, we investigated the labeling efficiency of hydrophilic (DAPI) and hydrophobic (Nile Red) fluorescent probes using uni- and bi-directional diffusion on porcine liver tissue sections on the order of a few hundred microns in thickness. Bi-directional diffusion significantly enhanced the penetration of probes. Although diffusion most accurately describes the labeling process, we found that after removal of the labeling molecules residual probe molecules in the tissue sections continued to be transported deeper into the tissue resulting in additional tissue constituents being labeled. Our study shows that bi-directional labeling can significantly enhance the labeling of thick tissues for applications in which structural information in three dimensions is needed.
- Subjects
HYDROPHILIC compounds; FLUORESCENT probes; FLUOROPHORES; MOLECULAR probes; MULTIPHOTON processes
- Publication
Microscopy Today, 2021, Vol 29, Issue 4, p22
- ISSN
1551-9295
- Publication type
Article
- DOI
10.1017/S1551929521000900