We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Dynamic Imaging of Marrow-Resident Granulocytes Interacting with Human Mesenchymal Stem Cells upon Systemic Lipopolysaccharide Challenge.
- Authors
Myers, Jay T.; Barkauskas, Deborah S.; Huang, Alex Y.
- Abstract
Human mesenchymal stem cells (hMSCs) have gained intense research interest due to their immune-modulatory, tissue differentiating, and homing properties to sites of inflammation. Despite evidence demonstrating the biodistribution of infused hMSCs in target organs using static fluorescence imaging or whole-body imaging techniques, surprisingly little is known about how hMSCs behave dynamically within host tissues on a single-cell level in vivo. Here, we infused fluorescently labeled clinical-grade hMSCs into immune-competent mice inwhich neutrophils andmonocytes express a second fluorescentmarker under the lysozyme M(LysM) promoter.Using intravital two-photon microscopy (TPM),we were able for the first time to capture dynamic interactions between hMSCs and LysM+ granulocytes in the calvarium bone marrow of recipient mice during systemic LPS challenge in real time. Interestingly, many of the infused hMSCs remained intact despite repeated cellular contacts with host neutrophils. However, we were able to observe the destruction and subsequent phagocytosis of some hMSCs by surrounding granulocytes. Thus, our imaging platformprovides opportunities to gain insight into the biology and therapeuticmechanisms of hMSCs in vivo at a singlecell level within live hosts.
- Subjects
GRANULOCYTES; MESENCHYMAL stem cells; LIPOPOLYSACCHARIDES; LYSOZYMES; NEUTROPHILS
- Publication
Stem Cells International, 2013, p1
- ISSN
1687-966X
- Publication type
Article
- DOI
10.1155/2013/656839