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- Title
Interaction of mercury(II) ions with immobilized apo-metallothioneins studied by scanning electrochemical microscopy combined with surface plasmon resonance.
- Authors
Xin, Ningning; Xin, Yinling; Gao, Yu; Xiang, Juan
- Abstract
Scanning electrochemical microscopy (SECM) was combined with surface plasmon resonance (SPR) and applied for in-situ monitoring of the incorporation of Hg by apo-metallothionein (apo-MT) immobilized on the SPR substrate. Hg was anodically stripped from the Hg-coated SECM Pt tip and sequestered by apo-MT upon its diffusion to the SPR substrate. The high sensitivity of the SPR instrument enabled the detection of the change in the composition and structure of apo-MT molecules that was induced by the metal sequestration of Hg. The SPR response revealed that the saturation co-ordination number of Hg binding to apo-MT was 18. Moreover, an unexpected collapse of the structure of MT was observed when the stoichiometric ratio of Hg/MT was ~70, and the structure cannot be further altered even by adding a large excess of Hg. This collapse was also confirmed by Raman spectroscopy. The results are potentially useful for a deeper understanding of the detoxification mechanism of MT to mercury ion. [Figure not available: see fulltext.]
- Subjects
MERCURY compounds; METALLOTHIONEIN; SCANNING electrochemical microscopy; SURFACE plasmon resonance; SEQUESTRATION (Chemistry); STOICHIOMETRY; CHEMICAL structure; RAMAN spectroscopy
- Publication
Microchimica Acta, 2011, Vol 174, Issue 1/2, p81
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-011-0598-z