We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cyclic GMP-linked pathway for renin secretion.
- Authors
Noble, Alan R.; Abu-Kishk, Rabee A.; D'Aloia, Marie-Ann E.; Williams, Brent C.; Lush, David J.
- Abstract
The role of cGMP as a second messenger for renin secretion is contentious. This was investigated using a superfused collagenase-dispersed rat kidney cortex cell preparation devoid of indirect influences on renin secretion. Nitroprusside, atriopeptin II and 8-Br-cGMP all increased renin release but the doseresponse relationships were biphasic. At low dose ranges there was a positive correlation between increasing drug concentration and renin secretion, but at high drug concentrations, a negative correlation was apparent. Methylene blue, a guanylate cyclase inhibitor, also suppressed baseline renin release at 10-5 and 10-6 M, but stimulated release at 10-3 M. Using mid-range drug concentrations, the cGMP specific phosphodiesterase inhibitor MB22948 potentiated renin release in response to nitroprusside and 8-Br-cGMP. Inhibition of guanylate cyclase with either methylene blue or LY83583 attenuated renin release in response to nitroprusside, but, as expected, had no effect on 8-Br-cGMP induced release. We conclude that, under physiological conditions, cGMP is a stimulatory second messenger for renin release. This activity is mimicked at low dose ranges by 8-Br-cGMP, nitroprusside and atriopeptin II. In response to high doses of these drugs an unknown inhibitory pathway is activated and this opposes, in a dose-related manner, the stimulatory actions of cGMP for renin release.
- Subjects
CYCLIC guanylic acid; RENIN; SECRETION; COLLAGENASES; KIDNEY cortex; LABORATORY rats
- Publication
Kidney International, 1994, Vol 46, Issue 6, p1588
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1994.454