We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rapamycin Enhances Repressed Autophagy and Attenuates Aggressive Progression in a Rat Model of IgA Nephropathy.
- Authors
Liu, Di; Liu, Yexin; Chen, Guochun; He, Liyu; Tang, Chengyuan; Wang, Chang; Yang, Danyi; Li, Huiqiong; Dong, Zheng; Liu, Hong
- Abstract
<bold>Background: </bold>IgA nephropathy (IgAN) has been considered to be the most frequent form of primary glomerulonephritis that occurs worldwide with a variety of factors involved in its occurrence and development. The impact of autophagy in IgAN, however, remains partially unclear. This study was designed to investigate the effects of rapamycin in an IgAN model.<bold>Method: </bold>After establishing an IgAN rat model, SD rats were divided into 4 groups: control, control + rapamycin, IgAN, IgAN + rapamycin. Proteinuria and the pathological changes and the level of autophagy of kidney were texted. Identify the expression of phosphorylation and total mammalian target of rapamycin (mTOR) and s6k1 as well as cyclin D1 in the kidney of rats through Western blot and immunohistochemistry.<bold>Results: </bold>With rapamycin treatment, we observed a significant reduction in the progression of proteinuria as well as alleviation of pathological lesions in IgAN rats. Besides, autophagy was inhibited, while the mTOR/S6k1 pathway was activated and expression of cyclin D1 was increased in IgAN. Rapamycin treatment increased autophagy and decreased the expression of cyclin D1.<bold>Conclusion: </bold>These results may suggest that mTOR-mediated autophagy inhibition may result in mesangial cell proliferation in IgAN.
- Subjects
PROTEIN metabolism; IMMUNOSUPPRESSIVE agents; RAPAMYCIN; AUTOPHAGY; ANIMALS; BIOLOGICAL models; CELL physiology; CELLULAR signal transduction; ELECTRON microscopy; GLOMERULONEPHRITIS; IMMUNOHISTOCHEMISTRY; KIDNEY glomerulus; PROTEINURIA; RATS; TRANSFERASES
- Publication
American Journal of Nephrology, 2017, Vol 45, Issue 4, p293
- ISSN
0250-8095
- Publication type
journal article
- DOI
10.1159/000456039