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- Title
Targeted Disruption of the Murine Homeodomain-Interacting Protein Kinase-2 Causes Growth Deficiency In Vivo and Cell Cycle Arrest In Vitro.
- Authors
Trapasso, Francesco; Aqeilan, Rami I.; Iuliano, Rodolfo; Visone, Rosa; Gaudio, Eugenio; Ciuffini, Laura; Alder, Hansjuerg; Paduano, Francesco; Pierantoni, Giovanna Maria; Soddu, Silvia; Croce, Carlo M.; Fusco, Alfredo
- Abstract
The homeodomain-interacting protein kinase 2 (HIPK2) protein is a member of a recently identified family of nuclear protein kinases that are well conserved in various organisms. HIPK2 can bind to several homeotic factors and to a series of proteins involved in the regulation of cell survival and proliferation in response to morphogenetic and genotoxic signals. Here we report Hipk2-targeted disruption in mouse; Hipk2−/− mice are viable and fertile but significantly smaller than their wild-type littermates. This feature is present at birth and retained throughout the mouse adulthood. Mouse embryo fibroblasts from Hipk2−/− mice show a reduced proliferation rate, compared to the wild-type counterparts, with accumulation in the G0/G1 phase of the cell cycle and altered levels of the cell cycle regulators cyclin D and CDK6. Restoration of wild-type HIPK2 expression in Hipk2−/− cells rescues the normal phenotype supporting a role for HIPK2 in the regulation of cell proliferation.
- Subjects
PROTEIN kinases; HOMEOBOX genes; CELLULAR control mechanisms; CELL cycle regulation; CELL proliferation; FIBROBLASTS
- Publication
DNA & Cell Biology, 2009, Vol 28, Issue 4, p161
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2008.0778