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- Title
Constitutive Expression of Interleukin-1beta (IL-1beta) in Rat Oligodendrocytes.
- Authors
Blasi, F.; Riccio, M.; Brogi, A.; Strazza, M.; Taddei, M.L.; Romagnoli, S.; Luddi, A.; DAngelo, R.; Santi, S.; Costantino-Ceccarini, E.; Melli, M.
- Abstract
The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1β mRNA, the type I IL-1 receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1β probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1β probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immuno-labelling technique utilized indicates coincidence of the signals on the brain slices. The results show that IL-1β mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1β. Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1β. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1β is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
- Publication
Biological Chemistry, 1999, Vol 380, Issue 2, p259
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/BC.1999.034