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- Title
Micropropagation and preparation of synthetic seed in M.26 apple rootstock I: Attempts towards saving labor in the production of adventitious shoot tips suitable for encapsulation.
- Authors
Sicurani, Melissa; Piccioni, Emanuele; Standardi, Alvaro
- Abstract
In order to achieve a time and hand-labor saving procedure for the use of direct organogenesis in the production of shoot tips suitable for encapsulation, a set of experiments aimed at the gradual reduction in the accuracy of selection, hand and machine excision of the explants (leaves and whole shoot clusters) was attempted. Vegetative performance of the regenerated shoots was evaluated and encapsulation and subsequent regrowth of adventitious shoot tips was performed. The research provided useful information to devise a mechanical protocol for the production of synthetic seed through encapsulation of differentiating propagules (tissue fragments with shoot primordia) in woody species.
- Publication
Plant Cell, Tissue & Organ Culture, 2001, Vol 66, Issue 3, p207
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1023/A:1010627720368