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- Title
Prevalencia de Escherichia coli, productora de BLEE en pacientes ambulatorios de la ciudad de Cuenca.
- Authors
Paúl Adrián, León Cajamarca; Gabriela Belén, Vázquez Guillén
- Abstract
A total of 274 urine samples were analyzed from outpatients presenting to Health Centers 1, 2 and 3 of the city of Cuenca during the period between May and June 2013, with the purpose to get at least 100 samples positive urine with Escherichia coli. Were retrieved 103 strains of Escherichia coli and the study was continued with the identification of the production of Extended Spectrum Beta Lactamases (ESBL), using the technique described in the CLSI manual. Were performed presumptive and confirmatory tests, applying the conventional method of agar diffusion plates in Mueller-Hinton agar, with a 0.5 McFarland inoculum and tested antimicrobial discs. For the presumptive test was used discs aztreonam, cefotaxime, ceftazidime and ceftriaxone, for the confirmatory test were used discs of ceftazidime and cefotaxime in combination with clavulanic acid. ESBL production was determined by the difference in the diameter of the halos as indicated in the technique. The results showed that of 103 strains of E. coli recovered seven (6,8 %) ESBL producing strains, when considering that the population which was worked was ofoutpatient, is very important the implementation of identification methods as ESBL for correct support of antimicrobial therapy, preventing this way the spread of strains of E. coli ESBL producing.
- Publication
INSPILIP. Revista Ecuatoriana de Ciencia, Tecnología e Innovacion en Salud Pública, 2019, Vol 3, Issue 1, p1
- ISSN
2588-0551
- Publication type
Article
- DOI
10.31790/inspilip.v2i2.61.g65