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- Title
High frequency of enterotoxin encoding genes of Staphylococcus aureus isolated from food and clinical samples.
- Authors
Haghi, Fakhri; Zeighami, Habib; Hajiloo, Zeynab; Torabi, Neda; Derakhshan, Safoura
- Abstract
<bold>Background: </bold>Staphylococcus aureus is recognized as an important cause of food poisoning related to the consumption of raw, undercooked, or mishandled foods worldwide.<bold>Methods: </bold>A total of 90 individual meat samples and 200 clinical specimens were collected and investigated the frequency of S. aureus and classical enterotoxin genes. The samples were cultured on Baird-Parker and Mannitol salt agar and subjected for confirmatory biochemical tests and molecular detection of femA, sea, seb, sec, sed, and see genes.<bold>Results: </bold>A total of 31 (34.5%) meat samples and 81 (40.5%) clinical specimens were positive for the presence of S. aureus. These isolates were detected with slightly higher frequency in clinical specimens than food samples (P> 0.05). Furthermore, the frequency of S. aureus in raw meat (23.4%) was higher than that in cooked meat samples (11.1%) (P< 0.05). Staphylococcal enterotoxin (SE) genes were identified in 18 (58.1%) of 31 meat isolates and 42 (51.8%) of 81 clinical isolates. The frequency of SE genes (except see) in meat isolates was slightly higher than that in clinical isolates (P> 0.05). We found sea and see genes with higher frequency than others in both meat and clinical samples. Furthermore, 55.5% of meat isolates and 38.1% of clinical isolates possessed more than one se gene.<bold>Conclusion: </bold>Detection of enterotoxigenic S. aureus in clinical and raw meat samples shows a probable risk for public health. Therefore, intensive and continuous monitoring of potentially pathogenic S. aureus is strongly recommended in order to evaluate the human health risk arising from food consumption.
- Subjects
STAPHYLOCOCCUS aureus; ENTEROTOXINS; FOOD poisoning; GENE frequency; FOOD consumption; CLOSTRIDIUM perfringens; STAPHYLOCOCCUS
- Publication
Journal of Health, Population & Nutrition, 2021, Vol 40, Issue 1, p1
- ISSN
1606-0997
- Publication type
journal article
- DOI
10.1186/s41043-021-00246-x