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- Title
T-cell costimulatory capacity of oral and skin epithelial cells in vitro: presence of suppressive activity in supernatants from skin epithelial cell cultures.
- Authors
Hasséus, B.; Jontell, M.; Bergenholtz, G.; Dahlgren, U. I.
- Abstract
Hasséus B, Jontell M, Bergenholtz G, Dahlgren UI. T-cell costimulatory capacity of oral and skin epithelial cells in vitro: presence of suppressive activity in supernatants from skin epithelial cell cultures. Eur J Oral Sci 2004; 112: 48–54.© Eur J Oral Sci, 2004 Oral Langerhans cells (LC) have better T-cell costimulatory capacity than skin LC. In this study factors affecting this capacity have been assessed in a mixed epithelial cell lymphocyte reaction (MELR) assay. Flow cytometry analysis of freshly recovered cells revealed major histocompatibility complex (MHC) class II molecule expression on 7.5% of the oral epithelial cells and 9.7% of the skin epithelial cells. Monoclonal anti class II antibodies significantly reduced the T-cell proliferation in the MELR. Pretreatment of skin epithelial cells with interleukin-1β, tumour necrosis factor-α or interferon (IFN)-γ did not affect the MELR proliferation, but incubation with IFNγ significantly suppressed the T-cell response. Transfer of supernatants from cultures of skin epithelial cells and allogeneic T cells to cultures of oral epithelial cells and T cells resulted in a reduced T-cell proliferation while supernatants from oral epithelial cells and T cells did not reduce proliferation. The higher proliferation in cultures of T cells and oral epithelial cells than in cultures containing skin epithelial cells may be due to the presence of a suppressive factor in the skin epithelial cell suspensions.
- Subjects
LANGERHANS cells; T cells; NEURAL stimulation; LYMPHOCYTES; EPITHELIAL cells; LYMPHOCYTE receptors
- Publication
European Journal of Oral Sciences, 2004, Vol 112, Issue 1, p48
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1111/j.0909-8836.2004.00097.x