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- Title
Vitamin D Reverses aPL-induced Inflammation and LMWH-induced s Flt-1 Release by Human Trophoblast.
- Authors
Gysler, Stefan M.; Mulla, Melissa J.; Stuhlman, Meredith; Sfakianaki, Anna K.; Paidas, Michael J.; Stanwood, Nancy L.; Gariepy, Aileen; Brosens, Jan J.; Chamley, Lawrence W.; Abrahams, Vikki M.
- Abstract
Problem Women with antiphospholipid syndrome ( APS) are at increased risk of recurrent pregnancy loss ( RPL) and preeclampsia. Antiphospholipid antibodies (a PL) directly alter trophoblast function. Treatment with low molecular weight heparin ( LMWH) reduces the risk of RPL but not preeclampsia. Moreover, LMWH stimulates trophoblast sFlt-1 release, an anti-angiogenic factor associated with preeclampsia. Since vitamin D deficiency is associated with APS and preeclampsia, this study sought to determine the effect of vitamin D on trophoblast function in the setting of a PL and LMWH. Method of study A human first trimester trophoblast cell line ( HTR8) and primary trophoblast cultures were treated with or without a PL in the presence and absence of vitamin D, LMWH or both. Trophoblast secretion of inflammatory cytokines and angiogenic factors were measured by ELISA. Results Vitamin D alone or in combination with LMWH attenuated the aPL-induced trophoblast inflammatory response in the HTR8 cells and primary cultures. While vitamin D did not have any impact on a PL-mediated modulation of angiogenic factors in the primary trophoblast, it significantly inhibited LMWH-induced s Flt-1 release. Conclusion LMWH in combination with vitamin D may be more beneficial than single-agent therapy by preventing a PL-induced trophoblast inflammation and reversing LMWH-induced s Flt-1 secretion.
- Subjects
ANTIPHOSPHOLIPID syndrome; RECURRENT miscarriage; PREGNANCY complications; IMMUNOLOGICAL aspects of pregnancy; HEPARIN; ENZYME-linked immunosorbent assay
- Publication
American Journal of Reproductive Immunology, 2015, Vol 73, Issue 3, p242
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12301