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- Title
Chloroplastic Phosphoadenosine Phosphosulfate Metabolism Regulates Basal Levels of the Prohormone Jasmonic Acid in Arabidopsis Leaves.
- Authors
Rodríguez, Victor M.; Chételat, Aurore; Majcherczyk, Paul; Farmer, Edward E.
- Abstract
Levels of the enzymes that produce wound response mediators have to be controlled tightly in unwounded tissues. The Arabidopsis (Arabidopsis thaliana) fatty acid oxygenation up-regulated8 (fou8) mutant catalyzes high rates of α-linolenic acid oxygenation and has higher than wild-type levels of the α-linolenic acid-derived wound response mediator jasmonic acid QA) in undamaged leaves. fou8 produces a null allele in the gene SAL1 (also known as FIERY1 or FRY1). Overexpression of the wild-type gene product had the opposite effect of the null allele, suggesting a regulatory role of SAL1 acting in JA synthesis. The biochemical phenotypes in fous were complemented when the yeast (Saccharomyces cerevisiae) sulfur metabolism 3' (2'), 5' -bisphosphate nucleotidase MET22 was targeted to chloroplasts in fou8. The data are consistent with a role of SAL1 in the chioroplast-localized dephosphorylation of 3' -phospho-5' -adenosine phosphosulfate to 5' -adenosine phosphosulfate or in a closely related reaction (e.g. 3' ,5' -bisphosphate dephosphorylation). Furthermore, the fou8 phenotype was genetically suppressed in a triple mutant (fou8 apk1 apk2) affecting chloroplastic 3'-phospho-5'-adenosine phosphosulfate synthesis. These results show that a nucleotide component of the sulfur futile cycle regulates early steps of JA production and basal JA levels.
- Subjects
ARABIDOPSIS thaliana; JASMONIC acid; LINOLENIC acids; SACCHAROMYCES cerevisiae; ADENINE nucleotides; GENE frequency; PHYSIOLOGY
- Publication
Plant Physiology, 2010, Vol 152, Issue 3, p1335
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1104/pp.109.150474