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- Title
Prostanoid F receptors elicit an inotropic effect in rat left ventricle by enhancing myosin light chain phosphorylation.
- Authors
Jon Riise; Cam H.T. Nguyen; Eirik Qvigstad; Dagny L. Sandnes; Jan-Bjørn Osnes; Tor Skomedal; Finn Olav Levy; Kurt A. Krobert
- Abstract
Aims The aims of this study were to determine if the prostanoid F receptor (FPR)-mediated inotropic effect in rat ventricle is mediated by increased phosphorylation of myosin light chain-2 (MLC-2) and to elucidate the signalling pathway(s) activated by FPRs to regulate MLC-2 phosphorylation. Methods and results Contractility was measured in left ventricular strips from adult male rats. Strips were also snap-frozen, and changes in the phosphorylation level of both MLC-2 and myosin phosphatase targeting subunit-2 (MYPT-2) were quantified. FPR stimulation with fluprostenol increased contractility by â¼100% above basal and increased phosphorylation of both MLC-2 (by â¼30%) and MYPT-2 (by â¼50%). The FPR-mediated inotropic effect and MLC-2 phosphorylation were reduced by a similar magnitude in the presence of the myosin light chain kinase (MLCK) inhibitor ML-7 (â¼60â70%) and an inhibitor of Ca2ﷆꖚ蝮阩, W-7 (â¼35%). Inhibition of Rho-associated kinase by Y-27632 reduced the FPR-mediated inotropic effect and MLC-2 phosphorylation by â¼40â45% and MYPT-2 phosphorylation by â¼70%. ML-7 and Y-27632 together reduced contractility and MLC-2 phosphorylation by â¼70â80%. The FPR-mediated inotropic effect was only modestly affected by high concentrations of the inositol tris-phosphate (IP3) receptor blocker 2-APB, but not by the protein kinase C (PKC) inhibitor bisindolylmaleimide. Conclusion The FPR-evoked inotropic effect is mediated by increasing the phosphorylation of MLC-2 through regulation of both MLCK and myosin light chain phosphatase activities. The second messenger IP3 and PKC are unlikely to be involved in the signalling cascade of the FPR-mediated positive inotropic effect. Therefore, FPR signalling mechanism(s) regulating MLC-2 phosphorylation likely extend beyond those classically established for Gq/11-coupled receptors.
- Subjects
PROSTANOIDS; LEFT heart ventricle diseases; MYOSIN; CARDIOTONIC agents; CELLULAR signal transduction; PHOSPHORYLATION; LABORATORY rats
- Publication
Cardiovascular Research, 2008, Vol 80, Issue 3, p407
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1093/cvr/cvn216