We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Análisis filogenético del virus Chikungunya en Colombia: evidencia de selección purificadora sobre el gen E1.
- Authors
Laiton-Donato, Katherine; Usme-Ciro, José A.; Rico, Angélica; Pardo, Lissethe; Martínez, Camilo; Salas, Daniela; Ardila, Susanne; Páez, Andrés
- Abstract
Introduction: Chikungunya virus (CHIKV) belonging to the Alphavirus genus, Togaviridae family, is a single-strand positive-sense RNA virus of 11,8 kb in length, transmitted by Aedes spp. Three genotypes have been identified worldwide: Asian, East/Central/South African (ECSA) and West African. Chikungunya Fever is an acute febrile disease mainly accompanied by polyarthralgia and cutaneous eruption. Following its introduction into The Americas in 2013, the first cases in Colombia occurred in September of 2014, reaching 399,932 cases in June of 2015. Objective: To identify the genotype or genotypes responsible for the current epidemic in Colombia and the viral genetic variability associated with the invasion of the country. Materials and Methods: Serum samples from selected patients with symptoms compatible with Chikungunya during 2014-2015 were selected for the study. RT-PCR of the E1 gene, sequencing, phylogenetic and adaptive evolution analyses were performed. Results: The study demonstrated exclusively the presence of the Asian genotype in Colombia. An average of 0.001 base substitutions per site, with 99.7% and 99.9% nucleotide identity and 99.9% amino acid identity was obtained among colombian sequences and other sequences from The Americas. The adaptive evolution analysis demonstrated that E1 gene is under strong purifying selection. Conclusions: The circulation of the Asian genotype of CHIKV was identified as the responsible for the first epidemic in Colombia. It is necessary to continue the genotypes surveillance in order to detect possible changes in epidemiology, viral fitness and pathogenicity.
- Publication
Biomédica: Revista del Instituto Nacional de Salud, 2016, Vol 36, p2
- ISSN
0120-4157
- Publication type
Article
- DOI
10.7705/biomedica.v36i0.2990