We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Phytochemical Analysis and Assessment of Antimicrobial, Genotoxic, and Cytotoxic Properties in Extracts Derived from Alyxia linearis Markgr. (Apocynaceae), a Philippine Endemic Species.
- Authors
Bautista, Gladys Ann B.; Garcellano, Rhea C.; Devanadera, Mark Kevin P.; Villaflores, Oliver B.; Aguinaldo, Alicia M.
- Abstract
The Apocynaceae is a known plant family that is rich in alkaloid, and many of its species were reported to have medicinal significance. An endemic plant from the Philippines, Alyxia linearis Markgr. -- which has not yet been investigated for its phytochemistry nor bioactivity -- is reported here. This study aims to characterize the phytochemical compounds and evaluate the antimicrobial, cytotoxic, and genotoxic activities of A. linearis. The hexane, dichloromethane, and methanol extracts from the roots, twigs, and leaves were subjected to different tests. The antimicrobial activity of the phytoconstituents was investigated against four bacteria (S. aureus, P. aeruginosa, E. faecalis, and E. coli) and two fungi (Penicillum sp. and A. niger). The antimicrobial analysis showed that among the nine extracts, only two extracts exhibited a partial inhibitory activity against those Gram-positive bacteria used, and only one extract exhibited a fungicidal activity. ATD inhibited the growth of both S. aureus and E. faecalis, whereas ARM inhibited only the growth of E. faecalis. Among the nine extracts, only ARD inhibited Penicillum growth. The genotoxicity of the extracts was tested using Allium cepa assay. The semipolar and polar extracts of A. linearis were all genotoxic except 1 ppm ALM. Cytotoxicity of the ALD extract was evaluated using an MTT assay on human dermal fibroblasts neonatal (HDFn). The assessment of cytotoxicity of ALD against HDFn showed an IC50 below 12.5 µg/mL as compared to the IC50 of the doxorubicin, which is 2.89 µg/mL. This indicates that the ALD extract is moderately cytotoxic as compared with doxorubicin. The phytochemical constituents of the A. linearis extracts were classified mainly as phenols, flavonoids, steroids, and triterpenes -- with a slight occurrence of coumarins, anthraquinones, and anthrones.
- Subjects
PHILIPPINES; ENDEMIC species; PHYTOCHEMICALS; APOCYNACEAE; ESCHERICHIA coli; ONIONS; ENDEMIC plants; ANTHRAQUINONES
- Publication
Philippine Journal of Science, 2024, Vol 153, Issue 3, p1005
- ISSN
0031-7683
- Publication type
Article
- DOI
10.56899/153.03.21