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- Title
Effects of Gui Zhi Ma Huang Ge Ban Tang on the TLR7 Pathway in Influenza Virus Infected Mouse Lungs in a Cold Environment.
- Authors
Qin, Hong-Qiong; Shi, Shan-Shan; Fu, Ying-Jie; Yan, Yu-Qi; Wu, Sha; Tang, Xiao-Long; Chen, Xiao-Yin; Hou, Guang-Hui; Jiang, Zhen-You
- Abstract
<italic>Objective</italic>. We wished to investigate the effects of the traditional Chinese medicine Gui Zhi Ma Huang Ge Ban Tang on controlling influenza A virus (IAV) infection and improving inflammation in mouse lungs.<italic> Method</italic>. Mice were maintained in normal and cold environments and infected with IAV by intranasal application, respectively. Real-time quantitative polymerase chain reaction was used to measure mRNA expression of TLR7, myeloid differentiation primary response 88 (MyD88), and nuclear factor-kappa B (NF-<italic>κ</italic>B)p65 in the TLR7 signaling pathway and virus replication in lungs. Western blotting was used to measure expression levels of TLR7, MyD88, and NF-<italic>κ</italic>B p65 proteins. Flow cytometry was used to detect the proportion of T-helper (Th)1/Th2 and Th17/T-regulatory (Treg) cells.<italic> Results</italic>. Application of Gui Zhi Ma Huang Ge Ban Tang in influenza-infected mice in a cold environment showed (i) downregulation of TLR7, MyD88, and NF-<italic>κ</italic>Bp65; (ii) inhibition of transcriptional activities of promoters coding for TLR7, MyD88, and NF-<italic>κ</italic>Bp65; (iii) reduction in the proportion of Th1/Th2 and Th17/Treg cells.<italic> Conclusions</italic>. Gui Zhi Ma Huang Ge Ban Tang had a good therapeutic effect on mice infected with IAV, especially in the cold environment. It could reduce lung inflammation in mice significantly and elicit an anti-influenza effect by downregulating expression of the key factors in TLR7 signaling pathway.
- Subjects
ANIMAL experimentation; COLD (Temperature); ECOLOGY; INFLUENZA; LUNG diseases; CHINESE medicine; MICE; POLYMERASE chain reaction
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2018, Vol 2018, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2018/5939720