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- Title
VİTİS VİNİFERA ÇEKİRDEK ÖZÜTÜ KULLANILARAK ALTIN NANOPARTİKÜLLERİN YEŞİL SENTEZİ VE KOLON KANSERİ (HT-29) HÜCRELERİNDE ANTİ-KANSER ÖZELLİKLERİNİN DEĞERLENDİRİLMESİ
- Authors
GENÇ, Selim; PEHLİVANOĞLU, Suray; AYDIN ACAR, Çiğdem; YEŞİLOT, Şükriye
- Abstract
Objective In recent years, metallic nanoparticles have attracted the attention in the field of nanomedicine with their antioxidant, antimicrobial and anticancer activities. Especially, metal nanoparticles produced by green synthesis method from medicinal plants with known pharmacological efficacy in cancer treatment have been spotlighted by researchers. Gold nanoparticles; become prominentamong metal nanoparticles with their unique chemical, physical and non-toxic properties. In this study, it was aimed to perform green synthesis of gold nanoparticles using Vitis vinifera (grape seed) aqueous extract and to evaluate cytotoxic, anti-proliferative and apoptotic effects of gold nanoparticles (Vv-AuNP) alone and with Gemcitabine co-administration on HT-29 human colon cancer cell line. Materials and Methods Green synthesis of gold nanoparticles was carried out using the aqueous extract of Vitis vinifera. Vv-AuNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, scanning electron microscope (SEM) and Energy Dispersive X-ray Spectroscopy (EDX). Vv-AuNPs were treated with HT-29 cells at a dose range of 0-80 µg / mL for 72 hours, alone and in combination with Gemcitabine (100 µg / mL or 200 µg / mL fixed dose). Cytotoxic effects were evaluated by MTT assay. In addition, the antiproliferative effects of Vv-AuNPs at increasing doses (0-400 µg / mL) alone and combined with Gemcitabine were determined by the BrdU test. After treatment of gold nanoparticles with HT-29 cells, the level of Caspase-3 activation was analyzed by Western-blot method. Results According to MTT results, when the dose of Vv-AuNPs was increased to 80 µg / mL, it was determined that HT-29 cells did not show the expected level of cytotoxic effect. According to the results of the BrdU cell proliferation test, IC50 values of Vv-AuNPs alone and combined with Gemcitabine were determined as 147.9 and 39.43 µg / mL, respectively. It was observed that the activation of caspase-3 due to Vv-AuNP doses increased compared to control, and its combined use with Gemcitabine reinforced this effect. Conclusion As a result, green synthesized AuNPs exhibited anti-cancer properties on colon cancer cells in vitro. The results of this study show that Vv-AuNPs can be considered as a potential option for colon cancer treatment.
- Publication
Medical Journal of Suleyman Demirel University, 2021, Vol 28, Issue 3, p455
- ISSN
1300-7416
- Publication type
Article
- DOI
10.17343/sdutfd.875236