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- Title
Characterization of the AP-1 μ1A and μ1B adaptins in zebrafish ( Danio rerio).
- Authors
Zizioli, Daniela; Forlanelli, Elena; Guarienti, Michela; Nicoli, Stefania; Fanzani, Alessandro; Bresciani, Roberto; Borsani, Giuseppe; Preti, Augusto; Cotelli, Franco; Schu, Peter
- Abstract
Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins γ1, β1, μ1, σ1. Mammalia express μ1A ubiquitously and isoform μ1B in polarized epithelia. Mouse γ1 or μ1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated μ1A and μ1B from Danio rerio. Analysis of μ1A and μ1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. μ1B transcript was detected in organs of endodermal derivation and 'knock-down' experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. μ1B is not expressed in murine liver, indicating loss of μ1B expression and establishment of alternative sorting mechanisms during mammalian development. Developmental Dynamics 239:2404-2412, 2010. © 2010 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2010, Vol 239, Issue 9, p2404
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.22372