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- Title
Overexpression of a Populus trichocarpa H<sup>+</sup>-pyrophosphatase gene PtVP1.1 confers salt tolerance on transgenic poplar.
- Authors
Yang, Y.; Tang, R. J.; Li, B.; Wang, H. H.; Jin, Y. L.; Jiang, C. M.; Bao, Y.; Su, H. Y.; Zhao, N.; Ma, X. J.; Yang, L.; Chen, S. L.; Cheng, X. H.; Zhang, H. X.
- Abstract
The Arabidopsis vacuolar H+-pyrophosphatase (AVP1) has been well studied and subsequently employed to improve salt and/or drought resistance in herbaceous plants. However, the exact function of H+-pyrophosphatase in woody plants still remains unknown. In this work, we cloned a homolog of type I H+-pyrophosphatase gene, designated as PtVP1.1, from Populus trichocarpa, and investigated its function in both Arabidopsis and poplar. The deduced translation product PtVP1.1 shares 89.74% identity with AVP1. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR analyses revealed a ubiquitous expression pattern of PtVP1.1 in various tissues, including roots, stems, leaves and shoot tips. Heterologous expression of PtVP1.1 rescued the retarded-root-growth phenotype of avp1, an Arabidopsis knock out mutant of AVP1, on low carbohydrate medium. Overexpression of PtVP1.1 in poplar (P. davidiana × P. bolleana) led to more vigorous growth of transgenic plants in the presence of 150 mM NaCl. Microsomal membrane vesicles derived from PtVP1.1 transgenic plants exhibited higher H+-pyrophosphatase hydrolytic activity than those from wild type (WT). Further studies indicated that the improved salt tolerance was associated with a decreased Na+ and increased K+ accumulation in the leaves of transgenic plants. Na+ efflux and H+ influx in the roots of transgenic plants were also significantly higher than those in the WT plants. All these results suggest that PtVP1.1 is a functional counterpart of AVP1 and can be genetically engineered for salt tolerance improvement in trees.
- Subjects
BLACK cottonwood; BALSAM poplar; INORGANIC pyrophosphatase; HYDROLASES; POPLARS
- Publication
Tree Physiology, 2015, Vol 35, Issue 6, p663
- ISSN
0829-318X
- Publication type
Article
- DOI
10.1093/treephys/tpv027