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- Title
PHYSICOCHEMICAL STUDIES OF DIFFERENT CALLI RAISED FROM LEAF EXPLANTS OF CATHARANTHUS ROSEUS.
- Authors
Gupta, Maneesh Kumar
- Abstract
Cancer is the abnormal growth of our body cells that can lead to death. Chemotherapy is used for the treatment of cancer, but due to high death rate and serious side effects of this therapy, the search for alternative method for treatment is still in progress. Catharanthus roseus; a renowned medicinal plant contains many powerful antitumor drugs such as vindoline, vincristine, vinblastine, serpentine, ajmalicine etc. for the treatment of different types of cancer. Biotechnological approaches specially plant tissue culture could be used for production of bioactive plant metabolites. In the present study the calli were developed on MS medium supplemented with 2, 4-D, NAA and Kn alone or in combination by using leaf and stem as explants from Catharanthus roseus plant. Efficient callus induction and growth was found on MS medium supplemented with 2mg/l NAA cultured in dark. The callus so obtained was also transferred on MS medium supplemented with different salts concentration (25mM, 50nM, 75mM and 100mM) keeping NAA concentration constant. In general the callus sub-cultured on MS medium supplemented with 2mg/l NAA was best in term of total crude alkaloid content when compared to different calli and plants parts.
- Subjects
CATHARANTHUS roseus; MEDICINAL plants; ANTINEOPLASTIC agents; PLANT metabolites; CALLUS (Botany)
- Publication
Plant Archives (09725210), 2019, Vol 19, Issue 2, p3063
- ISSN
0972-5210
- Publication type
Article