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- Title
Coronary Microembolization Induces Cardiomyocyte Apoptosis in Swine by Activating the LOX-1-Dependent Mitochondrial Pathway and Caspase-8-Dependent Pathway.
- Authors
Tao Liu; You Zhou; Jiang-You Wang; Qiang Su; Zhong-Li Tang; Yang-Chun Liu; Lang Li; Liu, Tao; Zhou, You; Wang, Jiang-You; Su, Qiang; Tang, Zhong-Li; Liu, Yang-Chun; Li, Lang
- Abstract
<bold>Background: </bold>Cardiomyocyte apoptosis by coronary microembolization (CME) contributes to myocardial dysfunction, in which mitochondrial pathway and death receptor pathway are activated. Lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) is a membrane protein involved in apoptosis. The study aimed to explore the role of LOX-1 in the activation of these 2 major apoptotic pathways.<bold>Methods: </bold>Twenty Bama miniature swine were randomized into 4 groups (n = 5 per group). The groups were Sham, CME, LOX-1 small-interfering RNA (siRNA), and control siRNA. Microspheres were injected into the left anterior descending artery of swine to establish CME model. Twelve hours after operation, cardiac function, serum c-troponin I level, microinfarct, and apoptotic index were examined. The levels of LOX-1, Bcl-2, Bax, cytochrome c as well as cleaved caspase 9, -8, and -3 were detected.<bold>Results: </bold>Myocardial dysfunction, enhanced serum c-troponin I, microinfarct, and apoptosis were induced following CME. Moreover, CME induced increased expression of LOX-1, Bax, cytochrome c, cleaved caspase 9, -8, and -3 as well as decreased Bcl-2 expression levels. The LOX-1 siRNA reversed these effects by CME except cleaved caspase 8 expression, while the control siRNA had no effect.<bold>Conclusion: </bold>Coronary microembolization induces cardiomyocyte apoptosis via the LOX-1-dependent mitochondrial pathway and caspase 8-dependent pathway.
- Subjects
HEART cells; APOPTOSIS; CASPASES; LOW density lipoproteins; SMALL interfering RNA; LABORATORY swine; CELL metabolism; PROTEIN metabolism; ANIMAL experimentation; BLOOD vessels; CELL receptors; CELLS; CELLULAR signal transduction; COMPARATIVE studies; RESEARCH methodology; MEDICAL cooperation; MITOCHONDRIA; RESEARCH; SWINE; THERAPEUTIC embolization; EVALUATION research
- Publication
Journal of Cardiovascular Pharmacology & Therapeutics, 2016, Vol 21, Issue 2, p209
- ISSN
1074-2484
- Publication type
journal article
- DOI
10.1177/1074248415599265